Soil degradation poses serious environmental problems and requires quantitative assessment. This study aimed to investigate the soil properties in rangelands with different management conditions. Based on the assessment of soil erosion, vegetation cover, vegetation composition, and vegetation vigor, 56 sites were selected in rangelands with moderate, poor, and very poor conditions located in 15 grazing-free areas and one wildlife refuge region. Soil samples (0–10 cm layer) were collected from these sites through a systematic-random method to compare their physical and chemical properties. Results showed that soil organic carbon (OC) and total nitrogen content were significantly different between sites with different range conditions. The mean weight diameter of water-stable aggregates and structural stability index in moderate rangelands were 1.56 mm and 3.98%, respectively, and declined towards more degraded rangeland (i.e. 0.84 mm and 0.83%). Grazing-free rangelands exhibited a relatively larger OC deficit; hence specific management measures and control of grazing are required to enhance the quality of soil in these sites. This study highlighted the role of organic matter in preserving soil structural stability. Increasing protection level of rangelands and decreasing grazing pressure may preserve soil quality and combat desertification in these arid regions.